homologous recombination

High Frequency of Homologous Recombination in the Genome of Modified Vaccinia Virus Ankara Strain ( MVA )

修饰的痘苗病毒安卡拉株(MVA)基因组中高频的同源重组 ( 英文 )

互联网摘选

Gene targeting is a rising technology in molecular biology, which is defined as the introduction of exogeneous DNA to specific site in genome by homologous recombination, and consequently change the hereditary character of the cell.

基因打靶技术是一项新兴的分子生物学技术，是利用外源DNA与受体细胞染色体DNA上的同源序列之间发生重组，并整合在预定位点上，从而改变细胞遗传特性的方法。

互联网摘选

Most members of the XRCC genes family participated in several DNA repair pathways, including base excision repair, homologous recombination repair and non-homologous end joining.

目前已鉴定的这一基因家族的多数成员均参与几种重要的DNA修复途径，包括碱基切除修复、同源重组修复和非同源末端重接。

互联网摘选

Mammalian cells accomplish DSBs via two pathways: non-homologous end joining and homologous recombination.

哺乳动物细胞有两种重要的DNA双链断裂修复方式：非同源末端连接和同源重组。

互联网摘选

High Frequency of Homologous Recombination in the Genome of Modified Vaccinia Virus Ankara Strain ( MVA)

修饰的痘苗病毒安卡拉株（MVA）基因组中高频的同源重组（英文）

互联网摘选

Homologous recombination ( HR) and the unusual origin end joining ( NHEJ) are two major pathways to repair DSB.

同源重组（HR）和非同源性末端连接（NHEJ）是DSB的两条重要修复途径。

互联网摘选

Scientists know that homologous recombination repair is also required for the repair of DNA cross-links, but the exact details remain to be resolved, Chen said.

科学家知道，DNA交联修复过程中也需要同源生物再结合修复参与，但是精确的细节仍有待研究。

互联网摘选

I mean using homologous recombination to knochout genes from genome. could you give me some advise?

是的,是用同源重组的方法使特定的基因片断缺失. 能否给点建议?

互联网摘选

There are complete repair system in normal cells, including base excision repair system ( BER) and homologous recombination repair system ( HR).

人类细胞内存在着完整的修复系统，其中包括碱基切除修复系统（base excision repair，BER）和同源重组修复系统（Homologous Recombination，HR）。

互联网摘选

Show that all transformants were homologous recombination transformants, but mixed with non-homologous recombinant transformants and hard to separate.

表明得到的转化子均为同源重组转化子，但其中混有非同源重组菌较难对其进行分离。

互联网摘选

In Archaea, it plays a critical role in homologous recombination process due to its RecA-like function.

在古细菌中，RadA蛋白具有RecA类似的功能并在同源重组过程中起关键作用。

互联网摘选

To disrupt a gene by homologous integration in Aspergillus fumigatus, transformation via the spheroplast method has been most widely used; this is a laborious and time-consuming method, and the homologous recombination frequency can be relatively low.

用原生质体通过同源重组来打断基因的方法曾经被广泛运用，但此方法费时、费力，而且其同源重组的频率也比较低。

互联网摘选

Knock out of bovine beta casein gene by homologous recombination

利用Red同源重组系统进行牛β酪蛋白基因敲除

互联网摘选

There are many DNA repair pathways to different DNA lesions, including base excision repair, nucleotide excision repair, mismatch repair and homologous recombination repair.

针对不同的DNA损伤。细胞内有许多不同的修复方式对其进行修复，包括碱基切除修复、核苷酸切除修复、错配修复、同源重组修复等。

互联网摘选

Results Homologous recombination between Alu repeats may result in various genetic exchanges, including duplications, deletions and translocations, cause a series of malignancy alteration by activating some oncogenes.

结果Alu序列之间的同源重组可导致基因缺失、重复，染色体移位导致基因重排，从而使某些癌基因活化，导致一系列的恶性改变。

互联网摘选

To explore the potential use of VTT as a live vaccine vector, we have generated three modified VTT strains ( MVTTs) by homologous recombination with the green fluorescent protein ( GFP) gene at three distinct genomic locations.

为了开发天坛株作为活载体来构建疫苗，我们通过同源重组的方法将绿色荧光蛋白基因GFP导入到其基因组的不同位置，构建了三种改良的痘苗病毒天坛株。

互联网摘选

Apart from DNA repair, non-homologous end joining ( NHEJ) may play a role in V ( D) J recombination, and integration of HIV-1 genome into a host genome, as well as the insertion of pseudogenes and repetitive sequences into the genome of mammalian cells.

非同源末端连接途径除了在DNA双链断裂修复中起重要作用外，在V（D）J重组、HIV-1病毒整合宿主基因，以及假基因和重复序列的插入上也起着重要作用。

互联网摘选